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cell through elisa  (R&D Systems)


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    R&D Systems cell through elisa
    <t>HB-EGF</t> increases early after the induction of MTLE in vivo and is secreted by NSPCs in vitro. (A) Confocal microscopy images from Nestin-GFP mice, 14dpKA showing the reduction caused by gefitinib of the MTLE-induced GCD and NSC overactivation. Scale bar, 20 μm. (B) Quantification of the volume of the GCL in saline+DMSO–, saline+ gefitinib–, KA+DMSO–, and KA+gefitinib–treated animals 14dpKA. (C) Quantification of the BrdU+ cells in saline+DMSO–, saline+ gefitinib–, KA+DMSO–, and KA+gefitinib–treated animals 14dpKA. (D) Quantification of BrdU+ NSCs, ANPs, and overall cells in the SGZ expressed as the percentage with respect to KA+DMSO animals. (E) Confocal microscopy images showing an increase in the EGFR ligand HB-EGF in the hilus and the molecular layer of Nestin-GFP mice 3dpKA. Scale bar, 20 μm. (F) Quantification of HB-EGF by <t>ELISA</t> at different time points during the first 3dpKA. Values are represented as the pg of HB-EGF in 100 μg of the tissue. (G) Immunofluorescence images of NSPCs in vitro showing HB-EGF expression. Scale bar, 10 μm. (H) Quantification by ELISA of HB-EGF released in vitro, expressed as the pg of the ligand per mL. Dots show individual data. Data information: for (B), Kruskal–Wallis test, * P < 0.05, * P < 0.01, and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (C), Kruskal–Wallis test, * P < 0.05, * P < 0.01, and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (D), t test, ** P < 0.01. Bars show the mean ± SEM. Dots show individual data. For (F), one-way ANOVA, * P < 0.05 and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (H), Kruskal–Wallis test, * P < 0.05. Bars show the mean ± SEM. Source data are available for this figure.
    Cell Through Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+through+elisa/pmc11231495-284-8-12?v=R%26D+Systems
    Average 94 stars, based on 11 article reviews
    cell through elisa - by Bioz Stars, 2026-08
    94/100 stars

    Images

    1) Product Images from "HB-EGF activates EGFR to induce reactive neural stem cells in the mouse hippocampus after seizures"

    Article Title: HB-EGF activates EGFR to induce reactive neural stem cells in the mouse hippocampus after seizures

    Journal: Life Science Alliance

    doi: 10.26508/lsa.202201840

    HB-EGF increases early after the induction of MTLE in vivo and is secreted by NSPCs in vitro. (A) Confocal microscopy images from Nestin-GFP mice, 14dpKA showing the reduction caused by gefitinib of the MTLE-induced GCD and NSC overactivation. Scale bar, 20 μm. (B) Quantification of the volume of the GCL in saline+DMSO–, saline+ gefitinib–, KA+DMSO–, and KA+gefitinib–treated animals 14dpKA. (C) Quantification of the BrdU+ cells in saline+DMSO–, saline+ gefitinib–, KA+DMSO–, and KA+gefitinib–treated animals 14dpKA. (D) Quantification of BrdU+ NSCs, ANPs, and overall cells in the SGZ expressed as the percentage with respect to KA+DMSO animals. (E) Confocal microscopy images showing an increase in the EGFR ligand HB-EGF in the hilus and the molecular layer of Nestin-GFP mice 3dpKA. Scale bar, 20 μm. (F) Quantification of HB-EGF by ELISA at different time points during the first 3dpKA. Values are represented as the pg of HB-EGF in 100 μg of the tissue. (G) Immunofluorescence images of NSPCs in vitro showing HB-EGF expression. Scale bar, 10 μm. (H) Quantification by ELISA of HB-EGF released in vitro, expressed as the pg of the ligand per mL. Dots show individual data. Data information: for (B), Kruskal–Wallis test, * P < 0.05, * P < 0.01, and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (C), Kruskal–Wallis test, * P < 0.05, * P < 0.01, and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (D), t test, ** P < 0.01. Bars show the mean ± SEM. Dots show individual data. For (F), one-way ANOVA, * P < 0.05 and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (H), Kruskal–Wallis test, * P < 0.05. Bars show the mean ± SEM. Source data are available for this figure.
    Figure Legend Snippet: HB-EGF increases early after the induction of MTLE in vivo and is secreted by NSPCs in vitro. (A) Confocal microscopy images from Nestin-GFP mice, 14dpKA showing the reduction caused by gefitinib of the MTLE-induced GCD and NSC overactivation. Scale bar, 20 μm. (B) Quantification of the volume of the GCL in saline+DMSO–, saline+ gefitinib–, KA+DMSO–, and KA+gefitinib–treated animals 14dpKA. (C) Quantification of the BrdU+ cells in saline+DMSO–, saline+ gefitinib–, KA+DMSO–, and KA+gefitinib–treated animals 14dpKA. (D) Quantification of BrdU+ NSCs, ANPs, and overall cells in the SGZ expressed as the percentage with respect to KA+DMSO animals. (E) Confocal microscopy images showing an increase in the EGFR ligand HB-EGF in the hilus and the molecular layer of Nestin-GFP mice 3dpKA. Scale bar, 20 μm. (F) Quantification of HB-EGF by ELISA at different time points during the first 3dpKA. Values are represented as the pg of HB-EGF in 100 μg of the tissue. (G) Immunofluorescence images of NSPCs in vitro showing HB-EGF expression. Scale bar, 10 μm. (H) Quantification by ELISA of HB-EGF released in vitro, expressed as the pg of the ligand per mL. Dots show individual data. Data information: for (B), Kruskal–Wallis test, * P < 0.05, * P < 0.01, and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (C), Kruskal–Wallis test, * P < 0.05, * P < 0.01, and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (D), t test, ** P < 0.01. Bars show the mean ± SEM. Dots show individual data. For (F), one-way ANOVA, * P < 0.05 and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (H), Kruskal–Wallis test, * P < 0.05. Bars show the mean ± SEM. Source data are available for this figure.

    Techniques Used: In Vivo, In Vitro, Confocal Microscopy, Saline, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Expressing



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    cell through elisa  (R&D Systems)
    94
    R&D Systems cell through elisa
    <t>HB-EGF</t> increases early after the induction of MTLE in vivo and is secreted by NSPCs in vitro. (A) Confocal microscopy images from Nestin-GFP mice, 14dpKA showing the reduction caused by gefitinib of the MTLE-induced GCD and NSC overactivation. Scale bar, 20 μm. (B) Quantification of the volume of the GCL in saline+DMSO–, saline+ gefitinib–, KA+DMSO–, and KA+gefitinib–treated animals 14dpKA. (C) Quantification of the BrdU+ cells in saline+DMSO–, saline+ gefitinib–, KA+DMSO–, and KA+gefitinib–treated animals 14dpKA. (D) Quantification of BrdU+ NSCs, ANPs, and overall cells in the SGZ expressed as the percentage with respect to KA+DMSO animals. (E) Confocal microscopy images showing an increase in the EGFR ligand HB-EGF in the hilus and the molecular layer of Nestin-GFP mice 3dpKA. Scale bar, 20 μm. (F) Quantification of HB-EGF by <t>ELISA</t> at different time points during the first 3dpKA. Values are represented as the pg of HB-EGF in 100 μg of the tissue. (G) Immunofluorescence images of NSPCs in vitro showing HB-EGF expression. Scale bar, 10 μm. (H) Quantification by ELISA of HB-EGF released in vitro, expressed as the pg of the ligand per mL. Dots show individual data. Data information: for (B), Kruskal–Wallis test, * P < 0.05, * P < 0.01, and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (C), Kruskal–Wallis test, * P < 0.05, * P < 0.01, and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (D), t test, ** P < 0.01. Bars show the mean ± SEM. Dots show individual data. For (F), one-way ANOVA, * P < 0.05 and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (H), Kruskal–Wallis test, * P < 0.05. Bars show the mean ± SEM. Source data are available for this figure.
    Cell Through Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+through+elisa/pmc11231495-284-8-12?v=R%26D+Systems
    Average 94 stars, based on 1 article reviews
    cell through elisa - by Bioz Stars, 2026-08
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    HB-EGF increases early after the induction of MTLE in vivo and is secreted by NSPCs in vitro. (A) Confocal microscopy images from Nestin-GFP mice, 14dpKA showing the reduction caused by gefitinib of the MTLE-induced GCD and NSC overactivation. Scale bar, 20 μm. (B) Quantification of the volume of the GCL in saline+DMSO–, saline+ gefitinib–, KA+DMSO–, and KA+gefitinib–treated animals 14dpKA. (C) Quantification of the BrdU+ cells in saline+DMSO–, saline+ gefitinib–, KA+DMSO–, and KA+gefitinib–treated animals 14dpKA. (D) Quantification of BrdU+ NSCs, ANPs, and overall cells in the SGZ expressed as the percentage with respect to KA+DMSO animals. (E) Confocal microscopy images showing an increase in the EGFR ligand HB-EGF in the hilus and the molecular layer of Nestin-GFP mice 3dpKA. Scale bar, 20 μm. (F) Quantification of HB-EGF by ELISA at different time points during the first 3dpKA. Values are represented as the pg of HB-EGF in 100 μg of the tissue. (G) Immunofluorescence images of NSPCs in vitro showing HB-EGF expression. Scale bar, 10 μm. (H) Quantification by ELISA of HB-EGF released in vitro, expressed as the pg of the ligand per mL. Dots show individual data. Data information: for (B), Kruskal–Wallis test, * P < 0.05, * P < 0.01, and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (C), Kruskal–Wallis test, * P < 0.05, * P < 0.01, and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (D), t test, ** P < 0.01. Bars show the mean ± SEM. Dots show individual data. For (F), one-way ANOVA, * P < 0.05 and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (H), Kruskal–Wallis test, * P < 0.05. Bars show the mean ± SEM. Source data are available for this figure.

    Journal: Life Science Alliance

    Article Title: HB-EGF activates EGFR to induce reactive neural stem cells in the mouse hippocampus after seizures

    doi: 10.26508/lsa.202201840

    Figure Lengend Snippet: HB-EGF increases early after the induction of MTLE in vivo and is secreted by NSPCs in vitro. (A) Confocal microscopy images from Nestin-GFP mice, 14dpKA showing the reduction caused by gefitinib of the MTLE-induced GCD and NSC overactivation. Scale bar, 20 μm. (B) Quantification of the volume of the GCL in saline+DMSO–, saline+ gefitinib–, KA+DMSO–, and KA+gefitinib–treated animals 14dpKA. (C) Quantification of the BrdU+ cells in saline+DMSO–, saline+ gefitinib–, KA+DMSO–, and KA+gefitinib–treated animals 14dpKA. (D) Quantification of BrdU+ NSCs, ANPs, and overall cells in the SGZ expressed as the percentage with respect to KA+DMSO animals. (E) Confocal microscopy images showing an increase in the EGFR ligand HB-EGF in the hilus and the molecular layer of Nestin-GFP mice 3dpKA. Scale bar, 20 μm. (F) Quantification of HB-EGF by ELISA at different time points during the first 3dpKA. Values are represented as the pg of HB-EGF in 100 μg of the tissue. (G) Immunofluorescence images of NSPCs in vitro showing HB-EGF expression. Scale bar, 10 μm. (H) Quantification by ELISA of HB-EGF released in vitro, expressed as the pg of the ligand per mL. Dots show individual data. Data information: for (B), Kruskal–Wallis test, * P < 0.05, * P < 0.01, and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (C), Kruskal–Wallis test, * P < 0.05, * P < 0.01, and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (D), t test, ** P < 0.01. Bars show the mean ± SEM. Dots show individual data. For (F), one-way ANOVA, * P < 0.05 and *** P < 0.001. Bars show the mean ± SEM. Dots show individual data. For (H), Kruskal–Wallis test, * P < 0.05. Bars show the mean ± SEM. Source data are available for this figure.

    Article Snippet: Next, we determined the amount of HB-EGF per cell through ELISA (Cat#DY8239-05; R&D Systems).

    Techniques: In Vivo, In Vitro, Confocal Microscopy, Saline, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Expressing